不同氨基酸组合对真鲷(Chrysophrys major)诱食效果的研究

试验以真鲷为试验对象,采用迷宫法,以丙氨酸、甘氨酸、精氨酸和组氨酸作为诱食剂原料,经不同组合后测定其对真鲷的诱食效果。结果显示,甘氨酸+组氨酸的诱食效果最好,其各种组合的诱食效果依次为甘氨酸+组氨酸>甘氨酸+丙氨酸>甘氨酸>组氨酸>丙氨酸>丙氨酸+精氨酸>丙氨酸+甘氨酸+组氨酸>精氨酸+组氨酸>甘氨酸+精氨酸>甘氨酸+丙氨酸+精氨酸>丙氨酸+组氨酸>精氨酸>丙氨酸+精氨酸+组氨酸>甘氨酸+丙氨酸+精氨酸+组氨酸。     

Caspase-9在肉用绵羊主要生殖器官中的分布研究

通过试验研究凋亡因子Caspase-9在肉用绵羊主要生殖器官中的表达,并探讨其生理意义.利用免疫组织化学的方法对Caspase-9在肉用绵羊主要生殖器官中的表达进行研究.研究结果显示,Caspase-9只在细胞质中表达,其在卵巢中主要分布于原始卵泡、初级卵泡及颗粒性黄体细胞,而卵泡膜性黄体细胞中未见表达;黄体期,主要表达于子宫的浅层腺体腺上皮细胞、子宫内膜上皮细胞、子宫颈黏膜固有层的淋巴小结、输卵管黏膜上皮分泌细胞和纤毛细胞、峡部的浆液性腺的腺上皮细胞;卵泡期,子宫和输卵管各个部位阳性反应不明显.在正常生理情况下,Caspase-9参与了肉用绵羊主要生殖器官周期性变化的调控和子宫黏膜免疫,对生殖器官功能的稳定发挥起着重要作用.     

Genetic variability of six indigenous goat breeds using major histocompatibility complex-associated microsatellite markers

The present study aimed at analyzing the genetic variability of indigenous goat breeds (Capra hircus) using the MHC-associated microsatellite markers BF1, BM1818, BM1258, DYMS1, and SMHCC1. The following breeds were included: Chinese Xuhuai, Indian Changthangi and Pashmina, Kenyan Small East African (SEA) and Galla, and Albanian Vendi. To examine genetic variability, the levels of heterozigosity, degrees of inbreeding, and genetic differences among the breeds were analyzed. The mean number of alleles ranged from nine in the Galla to 14.5 in the Vendi breed. The mean observed heterozygosity and mean expected heterozygosity varied from 0.483 in the Vendi to 0.577 in the Galla breed, and from 0.767 in the SEA to 0.879 in the Vendi breed, respectively. Significant loss of heterozygosity (p < 0.01) indicated that these loci were not in Hardy-Weinberg equilibrium. The mean F_IS values ranged from 0.3299 in the SEA to 0.4605 in the Vendi breed with a mean value of 0.3623 in all breeds (p < 0.001). Analysis of molecular variance indicated that 7.14% and 4.74% genetic variation existed among the different breeds and geographic groups, whereas 92.86% and 95.26% existed in the breeds and the geographic groups, respectively (p < 0.001). The microsatellite marker analysis disclosed a high degree of genetic polymorphism. Loss of heterozygosity could be due to genetic drift and endogamy. The genetic variation among populations and geographic groups does not indicate a correlation of genetic differences with geographic distance.     

上思县甘蔗主要虫害发生特点及防治对策

分析了上思甘蔗主要虫害发生原因及特点：主要是与当地气候条件关系密切,粗放的栽培管理方式与不合理的施药方法是导致虫害泛滥成灾的人为因素。提出了防治对策：通过科学的栽培管理方式与综合防治虫害措施,可达到趋利避害、高产优质的甘蔗生产目标。     

基于生态系统服务价值的宁夏区域生态补偿研究

采用宁夏回族自治区第二次土地调查和《宁夏统计年鉴2011》中的数据,运用相关公式,计算宁夏不同主体功能区和各县市区单位面积生态系统服务价值和生态补偿优先级(ECPS)。结果表明:宁夏限制开发生态区泾源、彭阳、隆德、红寺堡等县区单位面积生态系统服务价值和生态补偿优先级明显高于银川市辖区、石嘴山市辖区等重点开发区,后者应对前者进行生态补偿。     

玉米雄穗主轴长度和分枝数的主基因+多基因遗传分析

以PH4CV／昌7-2（组合Ⅰ）和PH6WC／7873（组合Ⅱ）的P1、P2、F1、F2、B1和B2六世代群体为材料,用主基因＋多基因六世代联合分离分析方法,研究了春播和夏播环境下雄穗主轴长度和雄穗分枝数的遗传规律。结果表明：2个组合雄穗主轴长在春播环境下均符合E-1模型。夏播环境下,组合Ⅰ雄穗主轴长符合C-0模型,组合Ⅱ雄穗主轴长符合E-3模型。在2个环境下,组合Ⅰ的雄穗分支数符合D一2模型,组合Ⅱ的雄穗分支数符合D-3模型。春播环境下,组合Ⅰ雄穗分支数表现为主基因遗传或以主基因遗传为主,主基因和多基因对2个组合雄穗主轴长的影响相当,可以采用单交重组或简单回交转育进行改良。夏播环境下,2个组合雄穗主轴长和组合Ⅱ雄穗分支数表现为多基因遗传或以多基因遗传为主,可以采用聚合回交或轮回选择累积增效基因的方法,以提高育种效率。     

Differences in Major Ions Composition of Artificial Seawater from Aquarium Tanks at the New Jersey State Aquarium

Concentrations of major seawater ions were monitored in six aquarium tanks at the New Jersey State Aquarium over a three-year period. The ratios of these ions to chlorinity were compared to those in freshly prepared artificial seawater. The largest aquarium tank (Ocean Tank) exhibits statistically significant (p<0.01) relative enrichment of potassium, calcium and strontium, and relative depletion of magnesium and sulphate. The likely source of excess potassium is from potassium iodide added to prevent goiter in sharks. Based on the excess potassium, a total amount of 650 μmol/kg iodide added over the years was calculated for the Ocean Tank. The excess calcium observed in several tanks correlates with the presence of concrete or arbonaceous shells in these tanks. In Ocean Tank, a calcium leaching rate of 6.7 kg/month was calculated. Continuous formation of white carbonaceous precipitates serves as a sink for magnesium in Ocean Tank, and a magnesium removal rate of 5.1 kg/month was calculated. These and other results show that concentrations of major ions in artificial seawater aquaria are affected by leaching, precipitation and addition of food supplements, and these factors should be taken into account when preparing artificial seawater for aquarium tanks with long water residence time. This revised version was published online in August 2006 with corrections to the Cover Date.     

Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIα and IIβ genes of Japanese flounder Paralichthys olivaceus

ABSTRACT:   The nucleotide sequences of Japanese flounder Paralichthys olivaceus , major histocompatibility complex (MHC) cDNA, classical MHC class Iα, non-classical MHC class Iβ, MHC class IIα and IIβ, were determined. The domain structures and antigen binding motifs of vertebrate MHC are conserved in the Japanese flounder MHC. A phylogenetic analysis supports the classification of these genes into class I and class II MHC. Classical MHC class Iα was ubiquitously expressed, whereas the non-classical MHC class Iβ was expressed mainly in lymphoid organs, gills, intestine and stomach. The MHC classes IIα and IIβ were also ubiquitously expressed.     

产学合作培养机械专业人才分析与实践——基于大学生就业视角

针对机械专业人才培养中存在的产学脱节、理论脱离实际和实践环节薄弱等问题,分析产学合作在机械专业学生培养中的作用.通过对机电工程学院近五年机械专业学生就业情况的分析,提出在产学合作的框架下,应调动企业的积极性,把机械行业对人才的要求贯彻到机械专业学生培养的教育理念、培养目标、办学机制、教学内容和方法等各个环节中,使机械专业人才的培养在产学合作中紧扣机械行业的发展趋势,实现培养优秀机械人才的目标.实践表明,产学合作极大地提高了学生的就业率和就业质量,可实现学校和企业"双赢",是一种有效的培养模式.